Immobilization of Phospholipase D for Production of Phosphatidylserine by a Pickering Emulsion Strategy

As a natural phospholipid, phosphatidylserine (PS) plays key role in the food, cosmetic, and pharmaceutical industries. Recently, substantial attention has been focused on phospholipase D (PLD)-mediated synthesis of PS. However, application free PLD is usually limited by high cost, poor reusability, low stability. In this study, from Streptomyces antibiotics (saPLD) was efficiently immobilized SiO2 through physical adsorption to develop saPLD@SiO2. The stability saPLD@SiO2 higher than that saPLD over an extensive range temperature pH conditions. Furthermore, PS yield approximately 41% first cycles, still kept 60% its initial after 14 cycles. After 25-day storage period, retained 62.5% activity, while only 34.3%, suggesting better saPLD. A Pickering emulsion produced dispersing solutions (ethyl propanoate acetate/acetic acid buffer) using ultrasound. engineered demonstrated excellent catalytic with 62% 6 h, had 18%. results indicated high-performance sustainable biocatalysis method established for effective